Rat Liver Adenosine Triphosphate:Adenosine Monophosphate Phosphotransferase Activity

Rat liver adenosine triphosphate: adenosine monophosphate phosphotransferase activity. II. Subcellular localization of adenylate kinase isozymes.

Adenylate kinase activity (ATP:AMP-phosphotransferase, EC 2.7.4.3) was studied in subcellular extracts of rat liver tissue. Essentially all of the activity, over 150 units per g of tissue, wet weight (1.7 units per mg of protein), was released into the cytosol when the tissue was homogenized in 0.025 M sucrose. Isotonic (0.25 M sucrose) homogenization and separation of subcellular fractions by ...

Adenosine triphosphate: adenosine monophosphate phosphotransferase isozymes in rat liver and hepatomas.

Adenosine Triphosphate : Guanosine Monophosphate Phosphotransferase

Several laboratories have reported studies on individual enzymes that belong to the general class of adenosine triphosphate : nucleoside monophosphate phosphotransferases (EC 2.7.4.4), commonly termed nucleoside monophosphate kinases (l-7). Studies with partially purified enzyme preparations have indicated that a variety of nucleoside monophosphates can serve as alternative substrates for nucle...

An adenosine 3':5'-monophosphate adenosine-binding protein from mouse liver. Association with S-adenosylhomocysteinase activity.

Cyclic AMP-binding proteins not associated with protein kinase activity have been described in various tissues [l-7 1. Most of these proteins have been shown to bind adenosine [3,6,7]. We have purified to apparent homogeneity a cyclic AMP adenosine-binding protein from mouse liver 273. The molecular properties of this protein and its interaction with adenosine, adenine and adenine nucleotides h...

Purification and properties of adenosine 5'-triphospae-D-glucose 6-phosphotransferase from rat liver.

1. An 870-fold purification of glucokinase from rat liver is described which involves ammonium sulphate fractionation and the use of DEAE-Sephadex, DEAE-cellulose and polyacrylamide columns. 2. The preparation is free of any interfering enzymes and has a specific activity of 8mumoles/min./mg. of protein. 3. Glucokinase catalyses the phosphorylation of glucose, mannose and 2-deoxyglucose. 4. The...